Partition of Transfer Ribonucleic Acid between Aqueous Salt Solutions and Trichlorotrifluoroethane Containing a Quaternary Amine
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منابع مشابه
Separation of methacrylic acid from aqueous phase using quaternary amine
The feasibility of extractive reaction of methacrylic acid from aqueous solution using a quaternary amine, tri–octyl methyl ammonium chloride (TOMAC) as an extractant, was studied. The diluents chosen in the present work belong to different chemical classes, n–butyl acetate, carbon tetrachloride, isoamyl alcohol, methyl isobutyl ketone, and toluene. The effect of initial acid concentration in t...
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Hybridization in 6xSSC (SSC, 0.15m-sodium chloride-0.015m-sodium citrate) at 66 degrees C was compared with hybridization in formamide-6xSSC (1:1, v/v) at 35 degrees C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridizati...
متن کاملseparation of methacrylic acid from aqueous phase using quaternary amine
the feasibility of extractive reaction of methacrylic acid from aqueous solution using a quaternary amine, tri–octyl methyl ammonium chloride (tomac) as an extractant, was studied. the diluents chosen in the present work belong to different chemical classes, n–butyl acetate, carbon tetrachloride, isoamyl alcohol, methyl isobutyl ketone, and toluene. the effect of initial acid concentration in t...
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The presence of dissolved salt during the enzymatic aminoacylation of transfer RNA diminishes the amount of aminoacyl-tRNA formed at the completion of the reaction. Experiments indicate that, although salt inhibits aminoacyltRNA synthetases, the diminution of the final level of aminoacylation is the result of salt-dependent changes in tRNA. tRNAs accepting different amino acids differ widely in...
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The formation and some properties of a stable complex between valyl transfer ribonucleic acid synthetase and transfer RNA from yeast have been investigated by three different methods for the isolation of the complex: titration over Sephadex, sucrose gradient centrifugation, and electrophoresis on Pevikon as supporting medium. Transfer RNA specific for valine (tRNAvsl) can be separated into two ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99751-6